In-Vitro Wound Contraction in the Horse: Differences Between Body and Limb Wounds
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This usually leads to the formation of exuberant granulation tissue even though these cells express a-smooth muscle actin (SMA).[6,7]
Cell viability remained high throughout the experiment, and there was no significant difference between the three cell types at 96 hours. Although there was no significant difference in the viability between the cell types, a reduction was observed in the NF and GT cells. This was probably due to contact inhibition as the gels contracted, causing some of the cells to die. At 96 hours, the contracted gels containing NF and GT cells were very densely populated.
There are anatomical and physiological differences seen in the horse when wound healing occurs. The problems with the distal limb may be attributed to poor blood supply, which in turn could lead to a deficit in nutrients and other cell mediators, which are needed for normal wound healing.
It is concluded from these studies that during second-intention wound healing in the horse, the differences in wound contraction between wounds on the limbs and the body are caused by differences in the contractile capacity of fibroblasts. The extracellular environment plays a role in the behavior of fibroblast cells. However, local environmental factors, such as the response to inflammation, may play a role in determining the rate of contraction during wound healing. The value of this work has been to show that the contractile variation of fibroblasts from different tissues is highest in the limb. The results do not match the observed wound contraction of in-vivo limb injuries, and, therefore, further research should be considered to determine those factors within the in-vivo limb environment that would allow the limb fibroblasts to contract to their observed full in-vitro potential.
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