Wound Healing Kinetics of the Genetically Diabetic Mouse
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Wild type versus diabetic wound healing kinetics. Delayed wound closure in db/db mice with a 1.5-cm2 full-thickness wound on the dorsum was observed after 4–6 weeks, compared to their nondiabetic counterparts, who healed in 10–16 days.65 After 2–3 weeks, db/db mice showed almost no signs of healing with wound contraction of 10%–20%, while wild type animals at the same time point were almost healed. Similarly, in another study using db/db mice,32 4-mm to 6.0-mm db/db wounds on the dorsum of each animal healed in 27.75 days ± 1.49.32,49
In previous studies that corroborate these results, different sizes of full-thickness wounds in the db/db showed significant delay compared to their wild-type counterparts.26
However, in the authors’ experience, using a 1.5 cm2 excisional wound in db/db mice showed slightly faster wound closure, reaching 34% after only 9 days. On the same day, wild type animals showed 84% wound closure (Figure 4).26,65
Wound closure kinetics of the db/db mouse. Considering the 2 main mechanisms of wound healing—contraction and re-epithelialization—db/db mice were found to heal mainly by re-epithelialization (Figure 5, Table 1) during the first 10 days of the follow-up, and contraction in the last 2 weeks. Although it has been reported that contraction is the main wound healing deficiency of this animal model,65,68 the authors of the present study found that it plays a main role to facilitate complete wound closure that starts at the second week of the follow-up period. On day 10, wounds displayed a peak in new epithelial formation from the wound edges.30,31,69
One cm2 full-thickness wounds on the back of the diabetic mice reached 50% wound closure in 13.7 ± 2.9 days.30,31 Diabetic mice with 0.6-, 1.0-, and 1.5-cm2 full-thickness wounds, regardless of initial wound size, demonstrated similar delays in wound healing.26 Ninety percent closure was reached in 19.9 ± 8.6 days post wounding by 1.0 cm2 wounds in the diabetic mice and consistently reached 80% wound closure by day 21 (Figure 5).30,31
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