Placenta Derived Adherent Cell (PDAC) Interaction and Response on Extracellular Matrix Isolated from Human Placenta

Mohit Bhatia, PhD; Christopher Lugo; Marian Pereira, PhD; Hemlata Rana; Sascha Abramson, PhD; Kristen Labazzo, PhD; Qing Liu, PhD;Wolfgang Hofgartner, MD, DSc; Robert Hariri, MD, PhD

The placenta is a rich source for proteins such as albumin,1 growth factors,2,3 and extracellular matrix proteins such as collagen4 and stem cells.5 This article describes the methods for the isolation of the whole placenta-derived extracellular matrix (pECM) tissue from the human placenta. The composition of the isolated matrix can be modified with slight changes in isolation methods.As the pECM is insoluble in water, this pECM material can be molded into water and cell culture media stable sheets and 3-dimensional (3-D) structures. Further, pECM-cell binding studies have been conducted to examine the cellular response of an adherent, pluripotent progenitor cell (PDACs) population isolated from human placenta (described as CD200+, CD105+, CD73+, CD34-, and CD45-).5

Based on the results presented in this work, one could envision a combination product composed of the pECM with PDACs. Such a 3-D mold may be useful for the treatment of nonhealing deep wounds, such as diabetic ulcers. The pECM would provide a support matrix for cells to attach to, proliferate and initiate cellular signaling and the PDACs would deliver key cytokines and chemokines to re-invigorate the wound healing process in nonhealing wounds.5


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