Nitric Oxide Synthase Activity and Angiogenesis Measured by Expression of CD34 in Burns Treated With Chitosan Films

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Author(s): 
Javier E. Ojeda, DVM, MS; Galo Cardenas, PhD ; Rodrigo Klassen, MD; Carlos von Plessing, PhD; Patricia Grez, MT; Jacqueline Sepulveda, PhD
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Chitosan enhances platelet adhesion and aggregation. Biochem Biophys Res Commun. 2003;302(3):480–483.

20. Kuo SM, Chang SJ, Chen T, Kuan T. Guided tissue regeneration for using a chitosan membrane: an experimental study in rats. J Biomed Mater Res A. 2006;76(2):408–415.

21. Ueno H, Murakami M, Okumura M, et al. Chitosan accelerates the production of osteopontin from polymorphonuclear leukocytes. Biomaterials. 2001;22(12):1667–1673.

22. Ueno H, Yamada H, Tanaka I, et al. Accelerating effects of chitosan for healing at early phase of experimental open wound in dogs. Biomaterials. 1999;20(15):1407–1414.

23. Schaffer MR, Efron PA, Thornton FJ, et al. Nitric oxide, an autocrine regulator of wound fibroblast synthetic function. J Immunol. 1997;158(5):2375–2381.

24. Kojima K, Okamoto Y, Kojima K, et al. Effects of chitin and chitosan on collagen synthesis in wound healing. J Vet Med Sci. 2004;66(12):1595–1598.

25. Diegelmann RF, Dunn JD, Lindblad WJ, Cohen IK. Analysis of the effects of chitosan on inflammation, angiogenesis, fibroplasia, and collagen deposition in polyvinyl alcohol sponge implants in rat wounds. Wound Repair Regen. 1996;4(1):48–52.

26. Kiyozumi T, Kanatani Y, Ishihara M, et al. The effect of chitosan hydrogel containing DMEM/F12 medium on full-thickness skin defects after deep dermal burn. Burns. 2007;33(5):642–648.

27. Rizk M, Witte MB, Barbul A. Nitric oxide and wound healing. World J Surg. 2004;28(3):301–306.

28. Lee RH, Efron D, Tantry U, Barbul A. Nitric oxide in the healing wound: a time-course study. J Surg Res. 2001;10(1):104–108.

29. Cals-Grierson MM, Ormerod A. Nitric oxide function in the skin. Nitric Oxide. 2004;10(4):179–193.

30. Mahoney E, Reichner J, Bostom LR, et al. Bacterial colonization and the expression of inducible nitric oxide synthase in murine wounds. Am J Pathol. 2002;161(6):2143–2152.

31. Carter EA, Derojas-Walker T, Tamir S, et al. Nitric oxide production is intensely and persistently increased in tissue by thermal injury. Biochem J. 1994;304(Pt 1):201–204.

32. Pan LR, Tang Q, Fu Q, et al. Roles of nitric oxide in protective effect of berberine in ethanol-induced gastric ulcer mice. Acta Pharmacol Sin. 2005;26(11):1334–1338.

33. Fukumura D, Gohongi T, Kadambi A, et al. Predominant role of endothelial nitric oxide synthase in vascular endothelial growth factor-induced angiogenesis and vascular permeability. Proc Natl Acad Sci U S A. 2001;98(5):2604–2609.

34. Lee PC, Salyapongse AN, Bragdon GA, Shears LL II, et al. Impaired wound healing and angiogenesis in eNOS-deficient mice. Am J Physiol. 1999;277(4 pt 2):H1600– H16008.

35. Müller A, Hermanns M, Skrzynski C, Nesslinger M, Müller KM, Kirkpatrick CJ. Expression of the endothelial markers PECAM-1, vWf, and CD34 in vivo and in vitro. Exp Mol Pathol. 2002;72(3):221–229.

Javier E. Ojeda, DVM, MS, Faculty of Veterinary Sciences, Universidad Austral de Chile; Galo Cardenas, PhD, Faculty of Chemistry Sciences, Universidad de Concepción, Chile; Rodrigo Klassen, MD and Patricia Grez, MT, Faculty of Medicine, Universidad de Concepción, Chile; Carlos von Plessing, PhD, Faculty of Pharmacy, Universidad de Concepción, Chile; Jacqueline Sepulveda, PhD, Faculty of Biological Sciences, Universidad de Concepción, Chile

Address correspondence to:
Javier E. Ojeda, DVM, MS
Institute of Clinical Veterinary Sciences
Faculty of Veterinary Sciences
Universidad Austral de Chile
Casilla 567
Valdivia, Chile
Email: javierojeda@uach.cl

Index: WOUNDS 2011;23(5):135–143