Abstract: The objective of the authors’ study was to assess the ability of autologous platelet lysate factors to facilitate healing of chronic cutaneous ulcers. Twenty-six patients with chronic leg ulcers participated in this prospective, randomized, double-blind, placebo-controlled trial at the wound care center at a tertiary care institution. Fifteen patients received autologous platelet lysate product mixed with collagen (treatment group); 11 patients received platelet-poor plasma mixed with collagen (placebo group). Treatment was applied twice daily for 12 weeks. After a two-week washout period, patients whose ulcers had not healed were assigned to receive the other treatment for an additional 12 weeks. The principal end point was the occurrence of complete healing (100% epithelialization of the entire target ulcer) as assessed by ulcer photography and clinical examination. A secondary end point was the rate at which the wound healed. Demographic characteristics were similar

Abstract: The optimal serum concentration for cryopreserving dermal fibroblasts has not been properly defined. Using flow cytometry, the authors determined whether human dermal fibroblasts from acute and chronic human wounds display differences in viability and apoptosis depending on the percent of fetal bovine serum (FBS) used for their cryopreservation. Fibroblast cultures were established from venous ulcers and from three-day-old acute wounds made on the thigh of each patient by punch biopsy. Cultures in their three to five in-vitro passage were cryopreserved for four weeks in Dulbecco’s Modified Eagle’s Medium (DMEM) plus dimethyl sulfoxide (DMSO, 10%) and 0-, 10-, 20-, 50-, and 80-percent FBS. After thawing, cells were allowed to attach to tissue culture plastic for four hours. Flow cytometry using propidium iodide (PI) to test for cell viability and Annexin V for early apoptosis was performed on attached cells and those still in the culture supernatant. Attached cells from acute

Abstract: The authors present a case of an isolated large ulceration on the palatal mucosa of a 48-year-old woman induced by methotrexate during the treatment of rheumatoid arthritis without psoriasis. The palatal ulcer was treated by topical wound care within six weeks. It is considered that multiple drug interactions and individual properties of patients may have a role in methotrexate toxicity. The histopathological mechanism of the mucocutaneous ulcerations secondary to methotrexate therapy may be multifactorial.