Objective. The aim of this cross-sectional study is to assess the epidemiological profile and antibiotic susceptibility of bacteria isolates in burn patients hospitalized from 2007 to 2015 in a Moroccan hospital. Materials and Methods. Of 627 samples, 451 wounds, 126 blood cultures, and 50 catheter samples were analyzed. A total of 610 microorganisms were isolated from a total of 108 patients. Results. The most prevalent isolates were Pseudomonas aeruginosa (18.5%), Acinetobacter baumannii (14.7%), and Staphylococcus aureus (14.5%); a high prevalence (31.1%) of methicillin-resistant S aureus also was found. Imipenem followed by piperacillin/tazobactam performed best against P aeruginosa. A baumannii isolates showed a very high resistance to imipenem and all beta-lactams antibiotics but were sensitive to colistin. Conclusions. The profile of this bacterial ecology shows there are resistant species requiring frequent use of second-line antibiotics and strengthening of aseptic procedures in burn treatments.